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 | Acesso ao texto completo restrito à biblioteca da Embrapa Agroenergia. Para informações adicionais entre em contato com cnpae.biblioteca@embrapa.br. |
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Registro Completo |
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Biblioteca(s): |
Embrapa Agroenergia; Embrapa Meio-Norte. |
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Data corrente: |
01/11/2022 |
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Data da última atualização: |
01/11/2022 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
FERREIRA, T. M. M.; FERREIRA FILHO, J. A.; LEAO, A. P.; SOUSA, C. A. F. de; SOUZA JUNIOR, M. T. |
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Afiliação: |
THALITA MASSARO MALHEIROS FERREIRA; JAIRE ALVES FERREIRA FILHO; ANDRE PEREIRA LEAO, CNPAE; CARLOS ANTONIO FERREIRA DE SOUSA, CPAMN; MANOEL TEIXEIRA SOUZA JUNIOR, CNPAE. |
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Título: |
Structural and functional analysis of stressinducible genes and their promoters selected from young oil palm (Elaeis guineensis) under salt stress. |
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Ano de publicação: |
2022 |
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Fonte/Imprenta: |
BMC Genomics, v. 23, n. 735, p. 1-13, 2022. |
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DOI: |
https://doi.org/10.1186/s12864-022-08926-6 |
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Idioma: |
Inglês |
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Conteúdo: |
Background Soil salinity is a problem in more than 100 countries across all continents. It is one of the abiotic stress that threatens agriculture the most, negatively affecting crops and reducing productivity. Transcriptomics is a technology applied to characterize the transcriptome in a cell, tissue, or organism at a given time via RNA-Seq, also known as full-transcriptome shotgun sequencing. This technology allows the identification of most genes expressed at a particular stage, and different isoforms are separated and transcript expression levels measured. Once determined by this technology, the expression profile of a gene must undergo validation by another, such as quantitative realtime PCR (qRT-PCR). This study aimed to select, annotate, and validate stress-inducible genes?and their promoters?differentially expressed in the leaves of oil palm (Elaeis guineensis) plants under saline stress. Results The transcriptome analysis led to the selection of 14 genes that underwent structural and functional annotation, besides having their expression validated using the qRT-PCR technique. When compared, the RNA-Seq and qRT-PCR profiles of those genes resulted in some inconsistencies. The structural and functional annotation analysis of proteins coded by the selected genes showed that some of them are orthologs of genes reported as conferring resistance to salinity in other species. There were those coding for proteins related to the transport of salt into and out of cells, transcriptional regulatory activity, and opening and closing of stomata. The annotation analysis performed on the promoter sequence revealed 22 distinct types of cis-acting elements, and 14 of them are known to be involved in abiotic stress. Conclusion This study has helped validate the process of an accurate selection of genes responsive to salt stress with a specific and predefined expression profile and their promoter sequence. Its results also can be used in moleculargenetics-assisted breeding programs. In addition, using the identified genes is a window of opportunity for strategies trying to relieve the damages arising from the salt stress in many glycophyte crops with economic importance. MenosBackground Soil salinity is a problem in more than 100 countries across all continents. It is one of the abiotic stress that threatens agriculture the most, negatively affecting crops and reducing productivity. Transcriptomics is a technology applied to characterize the transcriptome in a cell, tissue, or organism at a given time via RNA-Seq, also known as full-transcriptome shotgun sequencing. This technology allows the identification of most genes expressed at a particular stage, and different isoforms are separated and transcript expression levels measured. Once determined by this technology, the expression profile of a gene must undergo validation by another, such as quantitative realtime PCR (qRT-PCR). This study aimed to select, annotate, and validate stress-inducible genes?and their promoters?differentially expressed in the leaves of oil palm (Elaeis guineensis) plants under saline stress. Results The transcriptome analysis led to the selection of 14 genes that underwent structural and functional annotation, besides having their expression validated using the qRT-PCR technique. When compared, the RNA-Seq and qRT-PCR profiles of those genes resulted in some inconsistencies. The structural and functional annotation analysis of proteins coded by the selected genes showed that some of them are orthologs of genes reported as conferring resistance to salinity in other species. There were those coding for proteins related to the transport of salt into and out of cells, trans... Mostrar Tudo |
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Palavras-Chave: |
QRT-PCR; RNA-Seq. |
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Thesaurus Nal: |
Abiotic stress; Salinity; Transcriptomics. |
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Categoria do assunto: |
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X Pesquisa, Tecnologia e Engenharia |
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Marc: |
LEADER 02977naa a2200241 a 4500
001 2147944
005 2022-11-01
008 2022 bl uuuu u00u1 u #d
024 7 $ahttps://doi.org/10.1186/s12864-022-08926-6$2DOI
100 1 $aFERREIRA, T. M. M.
245 $aStructural and functional analysis of stressinducible genes and their promoters selected from young oil palm (Elaeis guineensis) under salt stress.$h[electronic resource]
260 $c2022
520 $aBackground Soil salinity is a problem in more than 100 countries across all continents. It is one of the abiotic stress that threatens agriculture the most, negatively affecting crops and reducing productivity. Transcriptomics is a technology applied to characterize the transcriptome in a cell, tissue, or organism at a given time via RNA-Seq, also known as full-transcriptome shotgun sequencing. This technology allows the identification of most genes expressed at a particular stage, and different isoforms are separated and transcript expression levels measured. Once determined by this technology, the expression profile of a gene must undergo validation by another, such as quantitative realtime PCR (qRT-PCR). This study aimed to select, annotate, and validate stress-inducible genes?and their promoters?differentially expressed in the leaves of oil palm (Elaeis guineensis) plants under saline stress. Results The transcriptome analysis led to the selection of 14 genes that underwent structural and functional annotation, besides having their expression validated using the qRT-PCR technique. When compared, the RNA-Seq and qRT-PCR profiles of those genes resulted in some inconsistencies. The structural and functional annotation analysis of proteins coded by the selected genes showed that some of them are orthologs of genes reported as conferring resistance to salinity in other species. There were those coding for proteins related to the transport of salt into and out of cells, transcriptional regulatory activity, and opening and closing of stomata. The annotation analysis performed on the promoter sequence revealed 22 distinct types of cis-acting elements, and 14 of them are known to be involved in abiotic stress. Conclusion This study has helped validate the process of an accurate selection of genes responsive to salt stress with a specific and predefined expression profile and their promoter sequence. Its results also can be used in moleculargenetics-assisted breeding programs. In addition, using the identified genes is a window of opportunity for strategies trying to relieve the damages arising from the salt stress in many glycophyte crops with economic importance.
650 $aAbiotic stress
650 $aSalinity
650 $aTranscriptomics
653 $aQRT-PCR
653 $aRNA-Seq
700 1 $aFERREIRA FILHO, J. A.
700 1 $aLEAO, A. P.
700 1 $aSOUSA, C. A. F. de
700 1 $aSOUZA JUNIOR, M. T.
773 $tBMC Genomics$gv. 23, n. 735, p. 1-13, 2022.
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Registro original: |
Embrapa Agroenergia (CNPAE) |
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| Acesso ao texto completo restrito à biblioteca da Embrapa Gado de Corte. Para informações adicionais entre em contato com cnpgc.biblioteca@embrapa.br. |
Registro Completo
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Biblioteca(s): |
Embrapa Gado de Corte. |
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Data corrente: |
15/03/2002 |
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Data da última atualização: |
15/03/2002 |
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Autoria: |
SCHENK, M. A. M.; MENDONCA, C. L.; MADRUGA, C. R.; KOHAYAWA, A.; ARAUJO, F. R. |
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Afiliação: |
Embrapa Gado de Corte (Campo Grande, MS). |
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Título: |
Avaliacao clinico-laboratorial de bovinos Nelore infectados experimentalmente com Trypanosoma vivax. |
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Ano de publicação: |
2001 |
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Fonte/Imprenta: |
Pesquisa Veterinaria Brasileira, Rio de Janeiro, v.21, n.4, p.157-161, out./dez. 2001. |
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Idioma: |
Português |
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Notas: |
CNPGC. |
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Conteúdo: |
Avaliaram-se as alteracoes clinico-laboratoriais de seis bezerros Nelore, de ambos os sexos, inoculados experimentalmente com 10 organismos viaveis de Trypanosoma vivax, isolados de bovinos da regiao de Pocone, Estado de Mato Grosso. Os animais foram observados diariamente, durante 30 dias, quanto aos parametros de temperatura retal, volume globular (VG), parasitemia, producao de anticorpos, coloracao de mucosas, comportamento e apetite. |
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Palavras-Chave: |
Brasil; Mao Grosso; Nelore; Pocone. |
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Thesagro: |
Bovino; Sanidade Animal; Trypanosoma Vivax. |
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Thesaurus NAL: |
animal health; Brazil; cattle; Nellore. |
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Categoria do assunto: |
-- |
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Marc: |
LEADER 01284naa a2200313 a 4500
001 1324418
005 2002-03-15
008 2001 bl uuuu u00u1 u #d
100 1 $aSCHENK, M. A. M.
245 $aAvaliacao clinico-laboratorial de bovinos Nelore infectados experimentalmente com Trypanosoma vivax.
260 $c2001
500 $aCNPGC.
520 $aAvaliaram-se as alteracoes clinico-laboratoriais de seis bezerros Nelore, de ambos os sexos, inoculados experimentalmente com 10 organismos viaveis de Trypanosoma vivax, isolados de bovinos da regiao de Pocone, Estado de Mato Grosso. Os animais foram observados diariamente, durante 30 dias, quanto aos parametros de temperatura retal, volume globular (VG), parasitemia, producao de anticorpos, coloracao de mucosas, comportamento e apetite.
650 $aanimal health
650 $aBrazil
650 $acattle
650 $aNellore
650 $aBovino
650 $aSanidade Animal
650 $aTrypanosoma Vivax
653 $aBrasil
653 $aMao Grosso
653 $aNelore
653 $aPocone
700 1 $aMENDONCA, C. L.
700 1 $aMADRUGA, C. R.
700 1 $aKOHAYAWA, A.
700 1 $aARAUJO, F. R.
773 $tPesquisa Veterinaria Brasileira, Rio de Janeiro$gv.21, n.4, p.157-161, out./dez. 2001.
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